Welcome to Phosphomatics V2 BETA!

Phosphomatics provides a suite of tools for the downstream analysis of mass spectrometry-based global phosphoproteomics data. Our goal is to provide statistical and biological context for the results generated by mass spectrometry and search software


This is the beta version of Phosphomatics version 2. We are making this resource public now to enable testing and refinement. It is still partially under development and frequent changes may be made without warning. If you notice bugs, please contact the developer
Currently, Phosphomatics V2 Beta works with Human phosphoproteomes only!
To use the previous version of Phosphomatics (associated with our 2020 Bioinformatics publication), click here.
Univariate and multivariate statistical analysis Pathway and ontology enrichment analysis
Substrate-kinase relation analysis Text mining and literature searching

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Upload your input data file below. Ensure that your file is correctly formatted.

Restore Previous

Enter the dataset ID from your previous analysis.


To help you explore the functionality of Phosphomatics, an example data set is provided. This is derived from Francavilla et al (2017) wherein an ovarian cancer cell line were treated in triplicate with either THZi1 (a CDK7 inhibitor), U0126 (a MEK inhibitor) or control.

An example Phosphomatics input file can be downloaded here. Alternatively, a shortcut link to a completed analysis of this data is provided below:


View the Phosphomatics documentation

Phosphomatics citations

Phosphomatics change log

Phosphomatics License


Phosphomatics is developed and maintained by the Melbourne Mass Spectrometry and Proteomics Facility at the Bio21 Institute, The University of Melbourne, Australia.

Updated: February 9th, 2022